Modulations of genes related to gut integrity, apoptosis, and immunity underlie the beneficial effects of Bacillus amyloliquefaciens CECT 5940 in broilers fed diets with different protein levels in a necrotic enteritis challenge model

Table 2 Sequences of primers used for quantitative real-time PCR

Gene	Accession No	Sequence (5'→3')	Size, bp	Annealing T, °C	Reference
CASP3	NM_204725.1	F-TGGTGGAGGTGGAGGAGC R- GTTTCTCTGTATCTTGAAGCACCA	110	62	This study
CASP8	NM_204592.2	F-GGAGCTGCTATCGGATCAAT R-GGAGCTGCTCTATCGGATCAAT	126	60	This study
IgA	S40610.1	F- GTCACCGTCACCTGGACACCA R- ACCGATGGTCTCCTTCACATC	192	64	[33]
IgG	X07174.1	F- ATCACGTCAAGGGATGCCCG R- ACCAGGCACCTCAGTTTGG	118	60	[34]
IgM	X01613.1	F- GCATCAGCGTCACCGAAAGC R- TCCGCACTCCATCCTCTTGC	98	60	[34]
MUC2	XM 001234581.3	F- CCCTGGAAGTAGAGGTGACTG R- TGACAAGCCATTGAAGGACA	143	60	[35]
OCLD	NM_205128.1	F- ACGGCAGCACCTACCTCAA R- GGGCGAAGAAGCAGATGAG	123	60	[36]
TJP1	XM_413773.4	F- GGATGTTTATTTGGGCGGC R- GTCACCGTGTGTTGTTCCCAT	187	60	[37]
CLDN1	NM_001013611.2	F- CTTCATCATTGCAGGTCTGTCAG R- AAATCTGGTGTTAACGGGTGTG	103	60	[37]
IL18	NM_204608.1	F- TGTGTGTGCAGTACGGCTTAG R- CTTACAAAAGGCATCGCATTC	79	60	[38]
Il-2	NM_204153.1	F- TCTGGGACCACTGTATGCTCT R- ACACCAGTGGGAAACAGTATCA	256	60	[39]
IFN-y	Y07922	F- AGCTGACGGTGGTGGACCTATTATT R- GGCTTTGCGCTGGATTC	259	60	[40]
ATP1A1	NM_205521.1	F- GTCAACCCGAGGGATGCTAA R- ACTGCTACAATGGCACCCTG	179	60	[41]
B⁰AT	XM_419056.5	F- GTGTTTGGAACCCTAAATACGAGG R- TAGCATAGACCCAGCCAGGA	72	60	[41]
GLUT2	NM_207178.1	F- TGATCGTGGCACTGATGGTT R- CCACCAGGAAGACGGAGATA	171	60	[41]
SI	XM_015291762.1	F- GCTTTAAGATGGGCAAGAGGAAG R- CCACCACCAGGCAAAAGAGG	65	60	[41]
HMBS	XM 417846.2	F- GGCTGGGAGAATCGCATAGG R- TCCTGCAGGGCAGATACCAT	131	60	[42]
YWHAZ	NM_001031343.1	F- TTGCTGCTGGAGATGACAAG R- CTTCTTGATACGCCTGTTG	61	60	[43]

ISSN: 2049-1891