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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Modulations of genes related to gut integrity, apoptosis, and immunity underlie the beneficial effects of Bacillus amyloliquefaciens CECT 5940 in broilers fed diets with different protein levels in a necrotic enteritis challenge model

Fig. 3

Effect of Bacillus amyloliquefaciens CECT 5490 (1.0 × 106 CFU/g of feed) (BA) on genes related to enzyme and nutrient transporters at day 16. (a-d) Effect of NE challenge and BA supplementation on the expression of two enzyme (ATPase Na+/K+ transporting subunit alpha-1 (ATP1A1) and sucrase-isomaltase (SI) and nutrient transporter genes (glucose transporter-2 (GLUT2) and neutral aminoacid transporter (B0AT) (e-d) Effect of crude protein (CP) level and BA supplementation on the expression of two enzyme (ATP1A1 and SI) and nutrient transporter genes (GLUT2 and B0AT). Non: non-challenged; NE: NE challenged; SCP: Standard protein diet; RCP: Reduced protein diet (2% lower than standard diets). Genes used as reference genes were HMBS and YWHAZ. Error bars show SEM for 8 replicates for each treatment

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