Fig. 6
From: Effects of miR-101-3p on goat granulosa cells in vitro and ovarian development in vivo via STC1
MiR-101-3p inhibits the expressions of cell proliferation-related genes via STC1. Goat granulosa cells are transfected with miR-101-3p mimics (miR-101-3p-mi) or mimics NC (NC-mi), miR-101-3p inhibitors (miR-101-3p-in) or inhibitors NC (NC-in). The (a) mRNA or (b) protein expressions of cell proliferation-related genes (CDK4, CCND1, CCNE1 and PCNA) are detected using RT-qPCR or Western blot after 24 h or 48 h transfection. Western blot measures the protein expressions of CDK4, CCND1, CCNE1 and PCNA in granulosa cells transfected with (c) siRNA-STC1 (si-STC1) or NC, pcDNA3.1-STC1 or pcDNA3.1 (control) or (d) miR-101-3p-mi or NC-mi or co-transfected miR-101-3p-mi with pcDNA3.1-STC1 for 48 h. β-actin is used as an internal control. Values are expressed as mean ± SD of n = 3. * P < 0.05, ** P < 0.01. Superscripts (a, b, c) show significant difference. NS means no significance