Fig. 5

MiR-101-3p inhibits granulosa cell proliferation via STC1. Goat granulosa cells are transfected with miR-101-3p mimics (miR-101-3p-mi) or mimics NC (NC-mi), miR-101-3p inhibitors (miR-101-3p-in) or inhibitors NC (NC-in), siRNA-STC1 (si-STC1) or NC, pcDNA3.1-STC1 or pcDNA3.1 (control). a Cell viability is standardized by relative absorbance (OD values at 450 nm) and determined using CCK8 assay at 12, 24, 48 and 72 h time points. After 24 h transfection, (b, c) granulosa cells in the S-phase are stained with EdU in red, whereas cell nuclei are dyed with DAPI in blue. The ratio of red and blue dyed cell numbers represents the percentage of EdU positive cells. Cell cycle distribution of granulosa cells is measured using flow cytometry. d The percentage of EdU positive cells or cell cycle distribution is detected using above-mentioned methods in granulosa cells transfected with miR-101-3p-mi or NC-mi or co-transfected miR-101-3p-mi with pcDNA3.1-STC1 for 24 h. Values are expressed as mean ± SD of n = 3. * P < 0.05, ** P < 0.01. Superscripts (a, b, c) show significant difference. NS means no significance