Table 1 Primer sequences used in this study (Sus scrofa)
From: MiR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine granulosa cells
Gene name | Forward | Reverse |
|---|---|---|
Cyclin B | 5’-AATCCCTTCTTGTGGTTA-3’ | 5’-CTTAGATGTGGCATACTTG-3’ |
Cyclin D | 5’-TACACCGACAACTCCATCCG-3’ | 5’-GAGGGCGGGTTGGAAATGAA-3’ |
Cyclin E | 5’-AGAAGGAAAGGGATGCGAAGG-3’ | 5’-CCAAGGCTGATTGCCACACT-3’ |
Star | 5’-CGTTTAAGCTGTGTGCTGGG-3’ | 5’-TCCATGACCCTGAGGTTGGA-3’ |
Cyp11a1 | 5’-GGGCAACCCATTTCCTACCA-3’ | 5’-CGAGCACTGGTGGTACAGAC-3’ |
Cyp19a1 | 5’-TCCGCAATGACTTGGGCTAC-3’ | 5’-GCCTTTTCGTCCAGTGGGAT-3’ |
Mfn2 | 5’-AGCGGCTGCGGTTTATC-3’ | 5’-TCTATATGGCGATGCAGTTCA-3’ |
NR5A1 | 5’-CTGCCTCAAGTTCCTCATTCTC-3’ | 5’-GGTAGTGGCACAGGGTGTAATC-3’ |
Gapdh | 5’-AGGTCGGAGTGAACGGATTTG-3’ | 5’-CCATGTAGTGGAGGTCAATGAAG-3’ |