Table 2 Primers used for real-time quantitative PCR
Gene | Accession No. | Primer sequences (5′→3′) | Size, bp |
|---|---|---|---|
ZO-1 | XM_005659811.1 | F: CAGCCCCCGTACATGGAGA | 114 |
R: GCGCAGACGGTGTTCATAGTT | |||
ZO-2 | NM_001206404.1 | F: ATTCGGACCCATAGCAGACATAG | 90 |
R: GCGTCTCTTGGTTCTGTTTTAGC | |||
OCLN | NM_001163647.2 | F: CTACTCGTCCAACGGGAAAG | 158 |
R: ACGCCTCCAAGTTACCACTG | |||
CLDN-1 | NM_001258386.1 | F: GCCACAGCAAGGTATGGTAAC | 140 |
R: AGTAGGGCACCTCCCAGAAG | |||
CLDN-2 | NM_001161638.1 | F: GCATCATTTCCTCCCTGTT | 156 |
R: TCTTGGCTTTGGGTGGTT | |||
GAPDH | NM_001206359.1 | F: TGAAGGTCGGAGTGAACGGAT | 114 |
R: CACTTTGCCAGAGTTAAAAGCA |