Table 1 Primer sequences for realtime PCR
Target genes | Primer sequences (5′ to 3′) | Products, bp | GenBank No. |
|---|---|---|---|
18S | F: CCCACGGAATCGAGAAAGAG R: TTGACGGAAGGGCACCA | 122 | AY265350.1 |
PPIA | F: GACTGAGTGGTTGGATGG R: TGATCTTCTTGCTGGTCTT | 116 | NM_214353.1 |
β-actin | F: CATCACCATCGGCAACGA R: GCGTAGAGGTCCTTCCTGATGT | 144 | XM_021086047.1 |
γ-actin | F: TCAGCAAGCAGGAGTACGAC R: GAGGTGTGTACATTTGCCAGG | 140 | XM_003357928.4 |
COX1 | F: TGGTGCCTGAGCAGGAATAGTG R: ATCATCGCCAAGTAGGGTTCCG | 88 | KF888634.1 |
COX2 | F: GCTTCCAAGACGCCACTTCAC R: TGGGCATCCATTGTGCTAGTGT | 154 | KF888634.1 |
COX3 | F: GGCTACAGGGTTTCACGGGTTG R: TCAGTATCAGGCTGCGGCTTCA | 130 | KF888634.1 |
ND1 | F: TCCTACTGGCCGTAGCATTCCT R: TTGAGGATGTGGCTGGTCGTAG | 165 | KF888634.1 |
ND2 | F: ATCGGAGGGTGAGGAGGGCTAA R: GTTGTGGTTGCTGAGCTGTGGA | 191 | KF888634.1 |
ND3 | F: AGCACGCCTCCCATTCTCAAT R: TGCTAGGCTTGCTGCTAGTAGG | 172 | KF888634.1 |
ND4 | F: TCGCCTATTCATCAGTAAGTCA R: GGATTATGGTTCGGCTGTGTA | 174 | KF888634.1 |
ND5 | F: CGGATGAGAAGGCGTAGGAA R: GCGGTTGTATAGGATTGCTTGT | 103 | KF888634.1 |
ND6 | F: ACTGCTATGGCTACTGAGATGT R: CTTCCTCTTCCTTCAACGCATA | 124 | KF888634.1 |
ND4L | F: GATCGCCCTTGCAGGGTTACTT R: CTAGTGCAGCTTCGCAGGCT | 182 | KF888634.1 |
ATP6 | F: ACTCATTCACACCCACCACACA R: CCTGCTGTAATGTTGGCTGTCA | 232 | KF888634.1 |
ATP8 | F: TGCCACAACTAGATACATCC R: GCTTGCTGGGTATGAGTAG | 107 | KF888634.1 |
CYTB | F: CTGAGGAGCTACGGTCATCACA R: GCTGCGAGGGCGGTAATGAT | 162 | KF888634.1 |