Fig. 6

Microscopic imaging analysis of TZPs and GJs in porcine oocytes. a Representative staning images of TZPs and GJs structures in oocytes, markered by F-actin and connexin 45, respectively; (b-e) Statistics of TZPs numbers (b) and the fluorescent intensities of TZPs (c), GJs (d) and their co-staining areas (e) in oocytes, markered by F-actin and connexin 45, respectively. Data are expressed as mean ± SEM. Control, ovaries were kept in 38.5 °C water bath for 1 h, and then the isolated COCs were cultured in 38.5 °C for 24 h; Heat, ovaries were kept in 41.5 °C water bath for 1 h, and then COCs were isolated and cultured in 38.5 °C for another 24 h. *P < 0.05 and **P < 0.01, compared with control (n = 30)