Fig. 3

G-actins gene and protein expressions in CCs after heat exposure. (a-b) Gene expression levels of β-actin and γ-actin in CCs; (c-d) Protein expression levels of β-actin and γ-actin in CCs. Data are expressed as mean ± SEM. Control, ovaries were kept in 38.5 °C water bath for 1 h, and then the isolated COCs were cultured in 38.5 °C for 0, 24 or 44 h; Heat, ovaries were kept in 41.5 °C water bath for 1 h, and then COCs were isolated and cultured in 38.5 °C for another 0, 24 or 44 h. ^{a, b, c, d} mean values not sharing the same letters are significantly different, P < 0.05 (n = 4)