Pharmacologic inhibition of mTORC1 mimics dietary protein restriction in a mouse model of lactation

Table 3 Free AA concentrations in refed dam mammary tissue on LD13^1,2

Amino acid	AP	PR		AP-R
	μmol/g protein	μmol/g protein	P-value³	μmol/g protein	P-value³	SEM
EAA
Arginine	1.98	3.57	0.61	1.88	0.98	1.91
Histidine	7.13	5.18	0.83	2.12	0.73	4.04
Isoleucine	4.31	4.84	0.82	4.50	0.97	1.05
Leucine	3.87	22.0	0.73	20.1	0.77	28.1
Lysine	14.7	16.8	0.88	17.8	0.83	5.40
Phenylalanine	2.09	2.88	0.69	1.58	0.84	1.10
Threonine	6.48	7.13	0.91	9.04	0.36	1.95
Valine	2.71	3.20	0.91	3.26	0.89	1.42
NEAA
Alanine	23.5	43.7	0.28	58.2	0.05	14.1
Aspartate	1.98	2.74	0.72	3.17	0.48	1.16
Glutamate	20.2	23.3	0.84	23.2	0.85	6.62
Glutamine	7.06	14.7	0.08	15.5	0.05	3.53
Proline	3.19	5.33	0.38	5.50	0.33	1.75
Serine	8.42	12.8	0.37	7.42	0.93	3.52
Tyrosine	5.39	5.67	0.97	6.25	0.81	1.64

¹AP = 18% crude protein diet, PR = 9% crude protein diet, AP-R = 18% crude protein diet coupled with rapamycin injections
²Data are means and pooled SEM, n = 6/treatment
³P-values correspond to difference between respective treatment and AP by ANOVA with Dunnett post hoc

ISSN: 2049-1891