Pharmacologic inhibition of mTORC1 mimics dietary protein restriction in a mouse model of lactation

Table 2 Free AA concentrations in refed dam plasma on LD13^1,2

Amino acid	AP	PR		AP-R
	μmol/L	μmol/L	P-value³	μmol/L	P-value³	SEM
EAA
Arginine	108	124	0.75	91	0.61	23.0
Histidine	105	117	0.73	116	0.79	19.1
Isoleucine	131	96	0.53	200	0.14	37.0
Leucine	265	57	0.19	70	0.22	114
Lysine	786	701	0.56	881	0.50	94.3
Methionine	96	59	0.51	132	0.51	37.0
Phenylalanine	89	71	0.27	82	0.79	12.0
Threonine	260	243	0.93	362	0.19	61.0
Tryptophan	64	50	0.75	76	0.78	22.0
Valine	224	141	0.21	325	0.11	51.2
NEAA
Alanine	631	974	0.12	1001	0.08	174
Aspartate	3.7	4.0	0.95	6.3	0.08	1.17
Glutamate	25	14	0.20	35.5	0.24	6.79
Glutamine	661	762	0.45	882	0.06	91.9
Proline	166	154	0.94	202	0.64	54.5
Serine	181	244	0.28	215	0.65	44.4
Tyrosine	292	185	0.31	387	0.38	78.7

¹AP = 18% crude protein diet, PR = 9% crude protein diet, AP-R = 18% crude protein diet coupled with rapamycin injections
²Data are means and pooled SEM, n = 6/treatment
³P-values correspond to difference between respective treatment and AP by ANOVA with Dunnett post hoc

ISSN: 2049-1891