Effects of supplementation of green tea extract on the milk performance of peripartal dairy cows and the expression of stress response genes in the liver

Table 6 Relative mRNA abundances of genes of the unfolded protein response (UPR) and genes of the Nrf2 pathway in the liver of cows fed the control diet (Con) or the diet supplemented with green tea extract (GTE) in weeks 1, 4 and 7 of lactation¹

	Week 1			Week 4			Week 7			P-value
	Con	GTE	SEM	Con	GTE	SEM	Con	GTE	SEM	GTE	Week	GTE × week	Parity
Genes of Nrf2 pathway²
CAT	1.00	1.02	0.18	1.11	0.92	0.18	1.41	1.23	0.18	0.15	0.003	0.49	0.036
GPX1	1.00	1.10	0.19	0.79^a	1.29^b	0.19	1.02	1.21	0.19	0.25	0.67	0.12	0.053
MT1A	1.00	0.71	0.32	0.19	0.35	0.16	0.10	0.15	0.32	0.85	< 0.001	0.37	0.81
NQO1	1.00	0.90	0.17	0.97	0.84	0.17	1.09	0.97	0.17	0.19	0.38	0.98	0.34
SOD1	1.00	0.89	0.10	0.95	0.85	0.10	1.00	0.92	0.10	0.08	0.48	0.96	0.16
UGT1A1	1.00	0.98	0.17	0.88	0.67	0.17	1.04	0.92	0.17	0.17	0.07	0.64	0.34
Genes of UPR²
ATF4	1.00	0.87	0.08	0.96	0.98	0.08	1.04	0.99	0.08	0.36	0.41	0.49	0.98
BAK1	1.00	1.32	0.32	0.76	1.27	0.32	0.90	1.06	0.32	0.19	0.61	0.71	0.11
BAX	1.00	0.94	0.19	1.49	1.43	0.19	1.12	1.25	0.19	0.71	0.001	0.71	0.12
CASP3	1.00^a	0.77^b	0.10	0.90	0.79	0.10	0.81	0.71	0.10	0.06	0.06	0.42	0.46
CASP8	1.00	0.76	0.14	1.07	1.13	0.14	0.97	1.01	0.14	0.82	0.017	0.10	0.28
DDIT3	1.00	0.73	0.14	0.97	1.15	0.14	0.96	0.91	0.14	0.96	0.051	0.028	0.08
DNAJC3	1.00^a	0.57^b	0.16	0.86	1.01	0.16	0.81	0.81	0.16	0.34	0.21	0.006	0.79
EDEM1	1.00	0.62	0.20	1.09	1.36	0.16	1.20	1.50	0.16	0.50	0.001	0.012	0.48
HERPUD1	1.00	0.89	0.16	0.69	0.77	0.16	0.76	0.76	0.16	0.79	0.10	0.66	0.48
HSPA5	1.00^a	0.57^b	0.20	0.62	0.67	0.20	0.60	0.59	0.20	0.20	0.32	0.11	0.47
PDIA4	1.00	0.64	0.19	0.75	0.93	0.19	0.93	0.82	0.19	0.31	0.91	0.10	0.49
XBP1s	1.00	0.61	0.21	0.33	0.68	0.21	0.42	0.49	0.21	0.87	0.046	0.055	0.19

¹Values are least square means, n = 15 for each group
²Relative mRNA abundances of Con in week 1 are set as a reference (=1.00)
^a,b Means within one week with different superscripts differ significantly (P < 0.05)
Nrf2 Nuclear factor-erythroid 2-related factor 2, SEM standard error of means, UPR unfolded protein response

ISSN: 2049-1891