Effects of supplementation of green tea extract on the milk performance of peripartal dairy cows and the expression of stress response genes in the liver

Table 5 Concentrations of acute phase and negative acute phase proteins in plasma and relative mRNA abundances of proinflammatory genes and genes of the acute phase reaction in the liver of cows fed the control diet (Con) or the diet supplemented with green tea extract (GTE) in weeks 1, 4 and 7 of lactation¹

	Week 1			Week 4			Week 7			P-value
	Con	GTE	SEM	Con	GTE	SEM	Con	GTE	SEM	GTE	Week	GTE × week	Parity
Plasma
SAA, μg/mL	0.33^a	0.26^b	0.02	0.33^a	0.27^b	0.02	0.29	0.25	0.02	0.007	0.029	0.52	0.41
HP, mg/mL	0.93	0.83	0.34	0.37	0.26	0.33	0.96	0.50	0.34	0.59	0.22	0.67	0.003
Albumin, g/dL	2.83	2.70	0.14	2.98	2.87	0.14	3.08	3.00	0.14	0.23	0.003	0.94	0.30
RBP4, μg/mL	1.59	1.66	0.15	1.57	1.64	0.15	1.76	1.76	0.15	0.22	0.08	0.87	< 0.001
Liver, relative mRNA abundances²
CCL2	1.00	1.02	0.31	1.21	1.13	0.31	1.53	1.56	0.31	0.90	0.034	0.96	0.68
IL1B	1.00	1.20	0.18	0.86	1.05	0.18	0.94	1.00	0.18	0.65	0.33	0.78	0.018
CXCL8	1.00	0.96	0.36	1.36	1.93	0.36	1.74	1.66	0.36	0.48	0.001	0.20	0.61
PTGS2	1.00	1.10	0.22	1.18	1.28	0.22	1.01	1.22	0.22	0.27	0.38	0.90	0.44
TNF	1.00	1.48	0.38	1.38	1.84	0.38	1.17	1.39	0.38	0.36	0.16	0.78	0.14
CP	1.00	0.81	0.16	0.72	0.77	0.16	0.84	0.75	0.16	0.66	0.23	0.42	0.49
CRP	1.00	0.65	0.16	1.21	1.04	0.16	1.16	0.93	0.16	0.035	0.030	0.72	0.87
HP	1.00	0.86	0.43	0.13	0.95	0.43	0.20	0.50	0.43	0.28	0.06	0.14	0.98
SAA3	1.00	1.29	0.36	0.54	0.79	0.36	0.58	0.63	0.36	0.46	0.025	0.83	0.93

¹Values are least square means, n = 15 for each group
²Relative mRNA abundances of Con in week 1 are set as a reference (=1.00)
^a,b Means within one week with different superscripts differ significantly (P < 0.05)
HP, haptoglobin; RBP4, retinol binding protein 4; SAA, serum amyloid A; SEM, standard error of means

ISSN: 2049-1891