| Item | Exp. 1 | Exp. 2 |
|---|
| Basal diet | FVS diet | FVS diet | N-free diet |
|---|
| Ingredients |
| Corn | 97.40 | 73.05 | – | – |
| Corn starch | – | – | 40.80 | 76.80 |
| Sucrose | – | – | 12.00 | 12.00 |
| Cellulose acetae | – | – | – | 4.00 |
| FVSa | – | 24.35 | 40.00 | – |
| Soybean oil | – | – | 3.00 | 3.00 |
| Dicalcium phosphate | 0.90 | 0.90 | 2.00 | 2.00 |
| Limestone | 0.90 | 0.90 | 0.80 | 0.80 |
| Sodium chloride | 0.30 | 0.30 | 0.50 | 0.50 |
| Choline chloride | – | – | 0.10 | 0.10 |
| Chromic oxide | – | – | 0.30 | 0.30 |
| Vitamin and mineral premixb | 0.50 | 0.50 | 0.50 | 0.50 |
| Analyzed nutrient levels |
| Dry matter | 86.29 | 86.81 | 89.66 | 89.23 |
| Crude protein | 7.52 | 7.95 | 6.01 | 0.76 |
| Organic matter | 97.07 | 94.98 | 93.17 | 94.67 |
| Essential amino acids, g/kg |
| Lysine | – | – | 2.20 | 0.08 |
| Methionine | – | – | 0.50 | – |
| Threonine | – | – | 2.00 | 0.19 |
| Tryptophan | – | – | 0.60 | – |
| Valine | – | – | 1.70 | 0.27 |
| Leucine | – | – | 5.20 | 0.50 |
| Isoleucine | – | – | 1.40 | 0.06 |
| Phenylalanine | – | – | 7.70 | 0.22 |
| Histidine | – | – | 0.80 | 0.17 |
| Arginine | – | – | 1.90 | 0.07 |
| Nonessential amino acids, g/kg |
| Tyrosine | – | – | 7.20 | 0.07 |
| Serine | – | – | 1.90 | 0.20 |
| Glutamic acid | – | – | 7.10 | 0.69 |
| Proline | – | – | 1.90 | 0.29 |
| Glycine | – | – | 1.70 | 0.23 |
| Alanine | – | – | 2.40 | 0.28 |
| Cysteine | – | – | 0.50 | – |
| Aspartic acid | – | – | 3.70 | 0.26 |
- aFVS: Flammulina velutipes stem waste
- bVitamin and mineral premix provided the following quantities of vitamins and minerals per kg of diet: vitamin A, 5512 IU; vitamin D3, 2200 IU; vitamin E, 30 IU; vitamin K3, 2.2 mg; vitamin B12, 27.6 μg; riboflavin, 4 mg; D-pantothenic acid, 14 mg; folic acid, 0.7 mg; thiamine, 1.5 mg; pyridoxine, 3 mg; biotin, 44 μg; Mn 40 mg (as manganese oxide); Fe, 75 mg (as iron sulfate); Zn, 75 mg (as zinc oxide); Cu, 100 mg (as copper sulfate); I, 0.35 mg (as potassium iodide) and Se, 0.3 mg (as sodium selenite)
