Fig. 6
From: Establishment of cell lines with porcine spermatogonial stem cell properties
Immortalized porcine SSCs display mutation in karyotype. a An immortalized porcine SSC with a normal karyotype (n = 38). b An immortalized porcine SSC with an abnormal karyotype (n = 35). Bar = 5 μm. c Percentages of immortalized porcine SSCs with different karyotype, calculated by cell numbers with the defined karyotype divided by the counted whole cell numbers. Data are presented as the mean ± SEM of three independent experiments. *: P < 0.05. d DNA content analysis of freshly isolated porcine germ cells (after differential plating), Ttag-transduced SSCs (passage 33) and Puro-transduced SSCs (passage 31)