Fig. 5

Ttag-transduced porcine SSCs colonize the recipient mouse testis. a The map of the vector pGreenPuro. b The bright (left) and fluorescent (right) images of Ttag-pGreenPuro-transduced cells (passage 20). Bar = 100 μm. c Hematoxylin & eosin (H&E) staining of testis sections from recipient mice 1 month after the busulfan treatment. Bar = 50 μm. d Visualization of the recipient seminiferous tubules (with/without transplantation of Ttag-pGreenPuro-transduced cells) under a bright (left) or fluorescent (middle) field. Bar = 50 μm. e The number of GFP⁺ cell colonies in the recipient testis. Data are presented as the mean ± SEM of four biological replicates (n = 4). **: P < 0.01. f The confocal microscopy analysis of the recipient seminiferous tubules (with or without transplantation of Ttag-pGreenPuro-transduced cells) showing the cell clusters co-expressing GFP and SV40. The dashed line delineates the putative basement membrane. Bar = 25 μm. g, h Staining of VASA and DAZL on dispersed seminiferous tubules g or on cryosections h from the testis transplanted with Ttag-transduced cells. Dashed ellipses refer to the transplanted cells settling down at the basement membrane. Bar = 20 μm