Fig. 5

Effect of CK2 inhibition on the acetylation level of α-tubulin in porcine oocytes. a Representative images of microtubule fibers before and after nocodazole treatment in control and CK2-inhibited oocytes. Oocytes were immunostained with anti-α-tubulin-FITC antibody to display microtubules and counterstained with PI to visualize chromosomes. Scale bar, 10 μm. b Representative images of acetylated α-tubulin in control and CK2-inhibited oocytes. Oocytes were immnunostained with anti-acetyl-α-tubulin (Lys-40) antibody to assess the acetylation level of α-tubulin. Scale bar, 5 μm. c The fluorescence intensity of acetylated α-tubulin was measured in control and CK2-inhibited oocytes. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. ***P < 0.001. d The acetylation levels of α-tubulin in control and CK2-inhibited oocytes were examined by western blotting. The blots were probed with anti-acetyl-α-tubulin (Lys-40) antibody and anti-Gapdh antibody, respectively