Fig. 4

Effect of CK2 inhibition on the spindle assembly and chromosome alignment in porcine oocytes. a Representative images of spindle morphologies and chromosome alignment in control and CK2-inhibited oocytes. M I oocytes were immnunostained with anti-α-tubulin-FITC antibody to visualize the spindles and counterstained with PI to visualize the chromosomes. Scale bar, 5 μm. b The rate of aberrant spindles was recorded in control and CK2-inhibited oocytes. c The rate of misaligned chromosomes was recorded in control and CK2-inhibited oocytes. Data of (b) and (c) were presented as mean percentage (mean ± SEM) of at least three independent experiments. ***P < 0.001