Fig. 6

RNA sequencing data Analysis for identifying differentially expressed genes among three groups (Control vs. 39 °C, Control vs. 40 °C and Control vs. 41 °C): DEGs in different comparisons in bGCs. a Graphical representation of significant DEGs disclosed among three comparison groups of bovine granulosa cells cultured under different intensities of heat stress. b Venn diagrams shows overlapping DEGs after heat stress among three comparisons. c. Heatmap of top 45 differentially expressed granulosa cell genes in heat stressed groups with FC > 2, P < 0.05. Red corresponds to up-regulated gene product, and green corresponds to down-regulated gene product. Each differentially expressed gene is represented by a single row, and each heat treatment group is represented by a single column