Fig. 3

a Effect of semen processing by SSRT method prior to cryopreservation on post thaw acrosome reactivity. The proportion of capacitation and acrosome-reacted, and uncapacitated spermatozoa were assessed by CTC assay. Each sample was incubated at 1 h, 8 h, and 25 h. Replicates of 2 for 192–297 sperm per replicate of SSRT and control sample per replicate. b Fluorescence photomicrography of sperms stained with CTC probe