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Table 2 Primers used for real-time quantitative PCR

From: Effects of dietary tributyrin on intestinal mucosa development, mitochondrial function and AMPK-mTOR pathway in weaned pigs

Primer namePrimer sequenceSize, bpAccession numbers
SGLT1F:5′-TCATCATCGTCCTGGTCGTCTC-3′
R:5′-CTTCTGGGGCTTCTTGAATGTC-3′
144M34044.1
GLUT2F:5′-ATTGTCACAGGCATTCTTGTTAGTCA-3′
R:5′-TTCACTTGATGCTTCTTCCCTTTC-3′
273NM_001097417
ASCT2F:5′-CTGGTCTCCTGGATCATGTGG-3′
R:5′-CAGGAAGCGGTAGGGGTTTT-3′
172DQ231578.1
y+LAT1F:5′-TTCTCTTACTCGGGCTGGGA-3′
R:5′-GCGCCATGAGACCATTGAAC-3′
400EU047705.1
PepT1F:5′-CAGACTTCGACCACAACGGA-3′
R:5′-TTATCCCGCCAGTACCCAGA-3′
99NM_214347.1
PGC-1αF:5′-CCCGAAACAGTAGCAGAGACAAG-3′
R:5′-CTGGGGTCAGAGGAAGAGATAAAG-3′
111NM_213963.2
TFAMF:5′-GGTCCATCACAGGTAAAGCTGAA-3′
R:5′-ATAAGATCGTTTCGCCCAACTTC-3′
167AY923074.1
NRF-1F:5′-GCCAGTGAGATGAAGAGAAACG-3′
R:5′-CTACAGCAGGGACCAAAGTTCAC-3′
166AK237171.1
β-actinF:5′-GGATGCAGAAGGAGATCACG-3′
R:5′-ATCTGCTGGAAGGTGGACAG-3′
130DQ845171.1
  1. SGLT1: sodium-glucose transporter 1; GLUT2: glucose transporter type 2; ASCT2: Na+-dependent neutral amino acid transporter 2; y+ LAT1: y+ L-type amino acid transporter 1; PepT1: dipeptide transporter 1; PGC-1α: peroxisomal proliferator-activated receptor-γ coactivator-1α; TFAM: mitochondrial transcription factor A; NRF-1: nuclear respiratory factor-1