Interactive association between processing induced molecular structure changes and nutrient delivery on a molecular basis, revealed by cutting-edge vibrational biomolecular spectroscopy

Table 4 Correlation between molecular structure related to amide region and predicted protein supply form combined feed (BPP; carinata meal and canola meal) using DVE system

Items		Amide I height	Amide II height	Amide I, II ratio	Amide I area	Amide II area	Amide area	α-helix height	β-sheet height	α-helix, β-sheet ratio
In situ ruminal degradation of CP^a
Kd, %/h	r	− 0.22	0.13	−0.53	− 0.36	0.12	− 0.19	0.02	− 0.09	0.25
Kd, %/h	P value	0.22	0.49	< 0.01	0.04	0.53	0.30	0.90	0.62	0.17
S, g/kg	r	0.23	0.67	−0.65	−0.03	0.59	0.18	0.44	0.32	−0.10
S, g/kg	P value	0.20	< 0.01	< 0.01	0.85	< 0.01	0.33	0.01	0.07	0.59
D, g/kg	r	0.35	0.24	0.12	0.40	0.25	0.38	0.44	0.47	−0.44
D, g/kg	P value	0.05	0.18	0.50	0.02	0.17	0.03	0.01	< 0.01	0.01
U, g/kg	r	−0.40	−0.57	0.32	−0.25	−0.50	− 0.36	−0.60	− 0.56	0.45
U, g/kg	P value	0.02	< 0.01	0.07	0.16	< 0.01	0.04	< 0.01	< 0.01	< 0.01
BCP, g/ kg DM	r	0.13	−0.35	0.58	0.34	−0.29	0.15	−0.17	− 0.03	− 0.14
BCP, g/ kg DM	P value	0.46	0.05	< 0.01	0.06	0.11	0.40	0.36	0.88	0.45
EDCP, g/ kg DM	r	0.65	0.62	−0.20	0.58	0.53	0.68	0.75	0.77	−0.64
EDCP, g/ kg DM	P value	< 0.01	< 0.01	0.26	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01
In vitro intestinal digestion of CP^b
dIDP, g/kg CP	r	−0.16	−0.58	0.55	−0.04	− 0.47	− 0.19	− 0.39	−0.38	0.16
dIDP, g/kg CP	P value	0.39	< 0.01	< 0.01	0.85	0.01	0.30	0.03	0.03	0.38
IDP, g/kg	r	0.18	−0.34	0.61	0.36	− 0.27	0.18	− 0.12	0.01	− 0.13
IDP, g/kg	P value	0.31	0.06	< 0.01	0.04	0.13	0.33	0.53	0.97	0.47
Total tract digestibility of CP^c
TDP, g/kg	r	0.02	−0.18	0.27	−0.02	−0.14	− 0.10	0.01	− 0.04	0.18
TDP, g/kg	P value	0.91	0.33	0.13	0.91	0.45	0.58	0.95	0.81	0.31
TDP, %	r	0.61	0.09	0.43	0.74	0.10	0.62	0.37	0.51	−0.54
TDP, %	P value	< 0.01	0.61	0.01	< 0.01	0.60	< 0.01	0.04	< 0.01	< 0.01

^aKd, degradation rate; S, soluble fraction in the in situ incubation; D, potentially degradable fraction; U, undegradable fraction, BCP, bypass crude protein; EDCP, effectively degraded of crude protein
^bdIDP, intestinal digestibility of rumen bypass protein on a percentage basis; IDP, intestinal digested crude protein
^cTDP, total digestion of crude protein

ISSN: 2049-1891