Bacillus sp. probiotic supplementation diminish the Escherichia coli F4ac infection in susceptible weaned pigs by influencing the intestinal immune response, intestinal microbiota and blood metabolomics

Table 3 Effect of dietary supplementation of Bacillus amyloliquefaciens DSM25840 and Bacillus subtilis DSM25841 on growth performance of weaned pigs challenged with Escherichia coli F4ac

Items	Treatments^a				SEM	P-value
Items	BAA	BAS	AB	CO	SEM	BAA vs. CO	BAS vs. CO	AB vs. CO
Body weight, g
Initial^b	7779	7789	7869	7619	266	0.60	0.60	0.49
Day 7^c	7638	7581	8208	7356	308	0.64	0.60	0.02
Day 14^d	8912	8279	9201	8370	350	0.33	0.92	0.05
Final (day 21)	10855	10036	11319	10102	549	0.28	0.94	0.03
Average daily live weight gain, g/d
Day 0 - day 7^c	−27.6	−41.6	48.5	−62.3	28.7	0.61	0.99	< 0.001
Day 7 - day 14^d	130	108	138	111	29.8	0.32	0.65	0.30
Day 14 - day 21^d	278	251	303	248	35.4	0.46	0.75	0.12
Post-challenge (day 8 - day 21)^d	204	179	220	179	29.1	0.27	0.63	0.09
Total (day 0 - day 21)^d	129	99	156	109	28.3	0.51	0.60	0.10
Average daily feed intake, g/d
Day 0 - day 7^c	162	163	200	146	15.6	0.70	0.34	< 0.001
Day 7 - day 14^d	308	254	376	318	46.8	0.78	0.27	0.32
Day 14 - day 21^d	535	532	612	519	47.1	0.75	0.71	0.07
Post-challenge (day 8 - day 21) ^d	421	393	494	418	44.6	0.99	0.65	0.11
Total (day 0 - day 21) ^d	329	318	396	330	33.3	0.82	0.72	0.06
Gain to feed
Day 0 - day 7^c	−0.52	−0.38	0.19	−0.56	0.19	0.62	0.76	< 0.001
Day 7 - day 14 ^d	0.38	0.37	0.23	0.34	0.11	0.54	0.61	0.57
Day 14 - day 21 ^d	0.51	0.46	0.49	0.47	0.03	0.35	0.93	0.39
Post-challenge (day 8 - day 21) ^d	0.46	0.44	0.44	0.42	0.03	0.06	0.11	0.22
Total (day 0 - day 21) ^d	0.36	0.28	0.38	0.30	0.05	0.22	0.74	0.20

^aBAA B. amyloliquefaciens DSM25840, BAS B. subtilis DSM25841, AB Antibiotic, CO Control
^bNumber of animals was 16 for BAA, BAS and CO groups; 15 for AB group
^cNumber of animals was: 14 for BAA; 15 for BAS, AB and CO.
^dNumber of animals was: 6 for BAA; 9 for BAS; 10 for AB; 6 for CO

ISSN: 2049-1891