Fig. 3

Putrescine rescued the proliferation and migration of LPS-treated IPEC-J2 cells. a Cells were seeded in 96-well plates, and pretreated for 48 h with or without 200 μmol/L putrescine. The cells were challenged with or without 100 μg/mL LPS for 4 h, and cell proliferation was measured with the EdU method. b IPEC-J2 cells were seeded in 6-well plates, and pretreated for 48 h with or without 200 μmol/L putrescine, followed by the addition of 2 μg/mL mitomycin C for 24 h. The cells were then challenged with or without 100 μg/mL LPS for 4 h before scratching. Images were taken immediately after scratching (0 h) and at 8 h post scratching to calculate area covered by cell migration. The scratched borders were enhanced with black lines. c Western blotting of phosphor-ERK1/2 and phospho-FAK. d Western blotting of total-Erk1/2 and total-FAK. e Western blotting of ODC. Values are means ± SE, n = 4. Means with different letters are different (P < 0.05). Ctrl, control; IPEC-J2, porcine intestinal epithelial cells; LPS, lipopolysaccharides; P-ERK1/2, phospho-ERK1/2; P-FAK, phospho-FAK; Put, putrescine