Fig. 1
From: Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression
SIRT1 subcellular re-localisation during oocyte meiosis. a SIRT1 in immature GV oocytes, with visible germinal vesicle (gv), and in vitro produced oocytes at different stages, i.e., NEBD (nuclear envelope breakdown), pre-MI (pre-metaphase I), MI, AITI (anaphase-telophase transition) and MII (metaphase II) oocytes with extruded polar body (pb). Scale bar represents 50 μm. b Negative control of immunostaining where the primary antibody was omitted. c Immunoblotting of SIRT1 (60–120 kDa) and β-actin (42 kDa), in different oocytes (GV, IVM, IVO), including the proportion of 60 and 65 kDa bands (min – max values are indicated). Approximately 200 lysed oocytes were loaded per lane. PP: pure SIRT1 protein. The full-length blot is presented in Additional file 1: Figure S1.3)