In vivo and in vitro protective effect of arginine against intestinal inflammatory response induced by Clostridium perfringens in broiler chickens

Zhang, Beibei; Gan, Liping; Shahid, Muhammad Suhaib; Lv, Zengpeng; Fan, Hao; Liu, Dan; Guo, Yuming

doi:10.1186/s40104-019-0371-4

Journal of Animal Science and Biotechnology

Table 2 Primers used for real-time PCR analysis^a

From: In vivo and in vitro protective effect of arginine against intestinal inflammatory response induced by Clostridium perfringens in broiler chickens

Gene name	Primer sequence (5′ to 3´)	Product size, bp	Efficiency, %	Accession number
CAT-1	F: ATGTAGGTTGGGATGGAGCC	280	113.556	XM_015277949.1
CAT-1	R: AACGAGTAAGCCAGGAGGGT
CAT-2	F: CAAGTCTTCTCGGCTCTAT	105	105.602	XM_015285435.1
CAT-2	R: GTGCCTGCCTCTTACTCA
CAT-3	F: CAAGACTGGCTCTGCCTACC	236	106.623	XM_015278426.1
CAT-3	R: GGATCAACGCAAAGAAGTCC
iNOS	F: TGGGTGGAAGCCGAAATA	241	103.087	NM_204961.1
iNOS	R: GTACCAGCCGTTGAAAGGAC
ARG2	F: GCCAACTGTACGACTTTGGAG	150	90.430	NM_001199704.1
ARG2	R: AGCTGTGTCCAGCAGCTACC
ADC	F: CCCAGTTTGAGGAGATTGCT	122	108.230	XM_004947849.2
ADC	R: AAAGGTGAAGGCTGAGGTGA
AGAT	F: TCGTCAAGAGGCCTGATCCA	140	111.489	XM_015291975.1
AGAT	R: CCAAGCCATAGGCGCTTCAA
IL-1β	F: ACTGGGCATCAAGGGCTA	131	104.419	XM_015297469.1
IL-1β	R: GGTAGAAGATGAAGCGGGTC
IL-6	F: CGCCCAGAAATCCCTCCTC	152	101.974	XM_015281283.1
IL-6	R: AGGCACTGAAACTCCTGGTC
IL-8	F: ATGAACGGCAAGCTTGGAGCTG	233	119.885	NM_205498.1
IL-8	R: TCCAAGCACACCTCTCTTCCATCC
IFN-γ	F: AGCTGACGGTGGACCTATTATT	259	108.556	NM_205149.1
IFN-γ	R: GGCTTTGCGCTGGATTC
IL-10	F: CGCTGTCACCGCTTCTTCA	88	98.435	NM_001004414.2
IL-10	R: TCCCGTTCTCATCCATCTTCTC
TGF-β3	F: CATCGAGCTCTTCCAGATCC	112	102.986	NM_205454.1
TGF-β3	R: GACATCGAAGGACAGCCACT
JAK1	F: TGCACCGTGACTTAGCAGCAAG	168	114.087	XM_015290965.1
JAK1	R: TCTGAATCAAGCATTCTGGAGCATACC
JAK2	F: TCGCTATGGCATTATTCG	197	110.029	XM_015280061.1
JAK2	R: GTGGGGTTTGGTCCTTTT
JAK3	F: GCATCCGCCGCCGTGTTG	108	96.164	NM_204996.3
JAK3	R: AGCACCGCAGCCTCTCCAG
STAT1	F: TAAAGAGGGAGCAATCAC	112	109.954	XM_015289392.1
STAT1	R: ATCAGGGAAAGTAACAGC
STAT6	F: GCAACCTCTACCCCAACA	127	101.256	XM_015274736.1
STAT6	R: TCCCTTTCGCTTTCCACT
GAPDH	F: TGCTGCCCAGAACATCATCC	142	98.200	NM_204305.1
GAPDH	R: ACGGCAGGTCAGGTCAACAA

^aAbbreviations: CAT Cationic amino acid transporter, iNOS inducible nitric oxide synthase, ARG2 Arginase 2, ADC Arginine decarboxylase, AGAT Arginine: glycine amidinotransferase, IFN-γ Interferon-γ, TGF-β3 Transforming growth factor-β3, JAK Janus kinase, STAT Signal transducer and activator of transcription, GAPDH Glyceraldehyde-3-phosphate dehydrogenase, F Forward, R Reverse

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ISSN: 2049-1891

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