In vivo and in vitro protective effect of arginine against intestinal inflammatory response induced by Clostridium perfringens in broiler chickens

Table 1 Diet composition and nutrient levels

Items	Control diet	l-arginine-supplemented diet
Ingredient, g/kg (unless otherwise indicated)
Corn	582.8	582.8
Soybean meal, 44% CP	291.1	291.1
Corn gluten meal	50.0	50.0
Soybean oil	25.0	25.0
Dicalcium phosphate	19.2	19.2
Limestone	8.9	8.9
Sodium chloride	3.0	3.0
Choline chloride, 50%	2.5	2.5
Mineral premix^a	2.0	2.0
d,l-Methionine, 98%	2.6	2.6
l-Lys-HCl, 98%	2.4	2.4
Vitamin premix^b	0.3	0.3
Ethoxyquin, 33%	0.2	0.2
l-Arg	0.0	4.0
l-Ala	10.0	6.0
Nutrient level^c, g/kg (unless otherwise indicated)
ME, Mcal/kg	2.98	2.98
CP	219.4	219.4
Ca	10.0	10.0
Non-phytate phosphorus	4.5	4.5
Lys, measured value	13.6	13.9
Met	5.9	5.9
Thr, measured value	8.6	8.8
Arg, measured value	14.2	17.2

^aSupplied per kilogram of complete feed: Mn, 100 mg; Fe, 80 mg; Zn, 75 mg; Cu, 8 mg; I, 0.35 mg; and Se, 0.15 mg
^bSupplied per kilogram of complete feed: vitamin A, 12,500 IU; vitamin D₃, 2500 IU; vitamin E, 30 IU; vitamin K₃, 2.65 mg; vitamin B₁, 2 mg; vitamin B₂, 6 mg; vitamin B₅, 12 mg; vitamin B₁₂, 0.025 mg; niacin, 50 mg; folic acid, 1.25 mg and biotin, 0.0325 mg
^cCalculated composition, unless otherwise indicated

ISSN: 2049-1891