GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm

Table 3 Correlation coefficients between relative GSTM3-abundances in both pre-frozen (P-F) and frozen thawed (F-T) spermatozoa and sperm quality parameters in pre-frozen (P-F) and frozen-thawed spermatozoa, evaluated 30 (F-T-30) and 240 (F-T-240) min post-thaw. *P < 0.05; **P < 0.01

		Relative levels of P-F GSTM3	Relative levels of F-T GSTM3
P-F	% Progressive motile spermatozoa	0.29	0.40
	% Total motile spermatozoa	0.21	0.31
	% SYBR-14⁺/PI⁻ spermatozoa	−0.23	−0.24
	% M540⁻/YO-PRO-1⁻ spermatozoa	− 0.11	−0.03
	% JC1_agg spermatozoa	0.06	0.09
	% E⁻/YO-PRO-1⁻ spermatozoa	−0.48	−0.27
F-T-30	% Progressive motile spermatozoa	−0.04	0.11
	% Total motile spermatozoa	−0.08	0.05
	% SYBR-14⁺/PI⁻ spermatozoa	−0.67*	−0.50
	% M540⁻/YO-PRO-1⁻ spermatozoa	−0.70*	− 0.54
	% JC1_agg spermatozoa	−0.80**	−0.70**
	% E⁻/YO-PRO-1⁻ spermatozoa	−0.74**	− 0.71**
F-T-240	% Progressive motile spermatozoa	0.08	0.19
	% Total motile spermatozoa	−0.08	0.04
	% SYBR-14⁺/PI⁻ spermatozoa	−0.75**	−0.64*
	% M540⁻/YO-PRO-1⁻ spermatozoa	−0.75**	− 0.71**
	% JC1_agg spermatozoa	−0.56	0.56
	% E⁻ / YO-PRO-1⁻ spermatozoa	−0.45	−0.40

ISSN: 2049-1891