Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli

Table 1 Ingredient compositions of experimental diets^a

Ingredient, %	Control diet
Corn	44.51
Dried whey	15.00
Soybean meal	14.00
Fish meal	10.00
Soy protein concentrate	7.00
Lactose	6.00
Soybean oil	2.00
Limestone	0.56
L-Lysine·HCl	0.15
DL-Methionine	0.06
L-Threonine	0.02
Salt	0.40
Vit-mineral, Sow 6^b	0.30
Total	100.00
Calculated energy and nutrients, as-fed
Metabolizable energy, kcal/kg	3487
SID Lysine, %	1.35
SID Methionine, %	0.44
SID Threonine, %	0.79
SID Tryptophan, %	0.23
SID Methionine and Cysteine, %	0.74
SID Leucine, %	1.68
SID Isoleucine, %	0.86
SID Valine, %	0.95
Analyzed nutrients, % as-fed
Crude protein, %	23.05
Ca, %	1.04
Total P, %	0.75

^aTwo additional diets were formulated by adding low (1.28 × 10⁹ CFU/kg) or high dose (2.56 × 10⁹ CFU/kg) of Bacillus subtilis (DSM 25841) to the control diet, respectively
^bProvided the following quantities of vitamins and micro minerals per kilogram of complete diet: Vitamin A as retinyl acetate, 11136 IU; vitamin D₃ as cholecalciferol, 2208 IU; vitamin E as DL-alpha tocopheryl acetate, 66 IU; vitamin K as menadione dimethylprimidinol bisulfite, 1.42 mg; thiamin as thiamine mononitrate, 0.24 mg; riboflavin, 6.59 mg; pyridoxine as pyridoxine hydrochloride, 0.24 mg; vitamin B₁₂, 0.03 mg; D-pantothenic acid as D-calcium pantothenate, 23.5 mg; niacin, 44.1 mg; folic acid, 1.59 mg; biotin, 0.44 mg; Cu, 20 mg as copper sulfate and copper chloride; Fe, 126 mg as ferrous sulfate; I, 1.26 mg as ethylenediamine dihydriodide; Mn, 60.2 mg as manganese sulfate; Se, 0.3 mg as sodium selenite and selenium yeast; and Zn, 125.1 mg as zinc sulfate

ISSN: 2049-1891