Dietary supplementation with free methionine or methionine dipeptide mitigates intestinal oxidative stress induced by Eimeria spp. challenge in broiler chickens

Table 3 Primer sequences used for RT-qPCR

Gene	Primer sequences (5′ → 3′)	Amplicon, bp^a	Accession No.
SOD1 ^b	F: AGATGGCAGTGGGAAATGAG	110	NM_205064.1
SOD1 ^b	R: ACTCAAGACAGCAGAGTAGTAATG
CAT	F: GAGGAACCCTCAGACTCATTTG	117	NM_001031215.2
CAT	R: CCATCAGGAATACCACGATCAC
PEPT1	F: CCCCTGAGGAGGATCACTGTT	66	KF366603.1
PEPT1	R: CAAAAGAGCAGCAGCAACGA
y ⁺ LAT-1	F: TGTTGGAGCCAGAGAAGGA	118	XM_418326.6
y ⁺ LAT-1	R: CACAAGGAGATAAAGCAAAGTC
B ⁰ AT1	F: TCTATTGAAGATTCGGGCAC	153	XM_419056.6
B ⁰ AT1	R: AATGGTAAGCACAAGGTATGG
CAT - 1	F: CGTGGCATCTCTGCTCATC	134	NM_001145490.1
CAT - 1	R: CTCCATCCCAACCTACATACTTA
TLR2	F: ACTGCCTGCAACGGTCAT	75	NM_204278.1
TLR2	R: CATCAGCTTCATTGTTGGTTTCTGT
TLR5	F: ACACGGCAATAGTAGCAACACATAT	91	XM_025148815.1
TLR5	R: ACACCTGGAACTTGGAAAAGAACAT
IL1B	F: GTCAACATCGCCACCTACAA	90	XM_015297469.1
IL1B	R: CGGTACATACGAGATGGAAACC
IL2	F: CCTCAAGAGTCTTACGGGTCTA	104	GU119890.1
IL2	R: AGTTGGTCAGTTCATGGAGAAA
IFNG	F: TGAGGTGATGTTTACCGAGTTT	94	NM_205149.1
IFNG	R: GCTTAGAGCTGAGCAGGTATG
LITAF	F: GAGCGTTGACTTGGCTGTC	64	NM_204267.1
LITAF	R: AAGCAACAACCAGCTATGCAC
CLDN1	F: ACTCCTGGGTCTGGTTGGT	100	AY750897.1
CLDN1	R: GACAGCCATCCGCATCTTCT
OCLN	F: ACGGCAGCACCTACCTCAA	123	D21837.1
OCLN	R: GGGCGAAGAAGCAGATGAG
iNOS	F: TCCTGAGTTCTGTGCCTTTG	92	U46504.1
iNOS	R: GTTCATCTCCTTCACCCACTG
ACTB	F: GCCAACAGAGAGAAGATGAC	130	L08165.1
ACTB	R: CACCAGAGTCCATCACAATAC

^abp Base pairs
^bSOD1 Superoxide dismutase 1 gene, CAT Catalase gene, PEPT1 Peptide transporter 1 gene, y⁺LAT-1 Neutral and cationic amino acid transporter 1 gene, B⁰AT1 System B⁰ neutral amino acid transporter 1 gene, CAT–1 Cationic amino acid transporter 1 gene, TLR2 Toll-like receptor 2 gene, TLR5 Toll-like receptor 5 gene, IL1B Interleukin 1 beta gene, IL2 Interleukin 2 gene, IFNG Interferon gamma gene, LITAF Lipopolysaccharide-induced tumor necrosis factor-alpha factor gene, CLDN1 Claudin-1 gene, OCLN Occludin gene, iNOS Inducible nitric oxide synthase gene, F Forward, R Reverse

ISSN: 2049-1891