Flutamide-induced alterations in transcriptional profiling of neonatal porcine ovaries

Table 1 Summary of data obtained from RNA-Seq of porcine neonatal ovaries

Sample	RNA Sequencing No.1						RNA Sequencing No.2
Sample	CTR1a	CTR2a	CTR3a	FLU1a	FLU2a	FLU3a	CTR1b	CTR2b	CTR3b	FLU1b	FLU2b	FLU3b
Number of row reads	25,218,729	24,265,954	26,786,205	29,657,600	31,499,003	33,066,267	25,684,749	29,793,840	24,982,931	26,909,112	26,958,364	29,883,752
Number of processed reads	22,946,350	22,025,084	23,919,783	26,265,118	28,431,555	29,051,215	24,815,278	28,489,892	23,409,293	25,573,491	25,420,566	28,108,743
% of processed reads	90.99	90.77	89.30	88.56	90.26	87.86	96.61	95.62	93.70	95.04	94.30	94.06
Unique reads
Number of uniquely mapped reads	16,920,873	15,737,185	17,549,085	19,080,540	20,774,178	20,886,099	19,415,040	22,389,585	18,221,092	20,157,124	19,841,031	21,895,703
% of uniquely mapped reads	73.74	71.45	73.37	72.65	73.07	71.89	78.24	78.59	77.84	78.82	78.05	77.90
Average mapped length, nt	176.97	176.39	177.02	176.83	176.94	176.78	177.53	177.65	177.56	177.60	177.68	177.56
Multi-mapping reads
Number of reads mapped to multiple loci	1,728,713	1,588,546	1,774,327	1,896,456	2,078,804	2,120,266	1,905,286	2,239,355	1,818,916	1,987,466	1,973,831	2,205,331
% of reads mapped to multiple loci	7.53	7.21	7.42	7.22	7.31	7.30	7.68	7.86	7.77	7.77	7.76	7.85
Identified transcripts
Number of identified transcripts	43,478	43,702	41,124	42,171	43,952	43,531	42,672	44,969	40,724	41,812	43,259	43,307
% of identified transcripts	71.85	72.22	67.96	69.69	72.63	71.93	70.51	74.31	67.29	69.09	71.48	71.56

FLU ovaries from flutamide-treated piglet, CTR ovaries from NaCl-treated piglet
Each biological sample was sequenced two times (for details please see the M & M section)
Sequencing No. 1 (CTR1a, CTR2a, CTR3a, FLU1a, FLU2a and FLU3a) and Sequencing No. 2 (CTR1b, CTR2b, CTR3b, FLU1b, FLU2b and FLU3b) produced two
separate sets of data. The statistics are presented for each sample separately
Preprocessing of data included clipping of adapters, trimming of read ends and removing low quality reads
“Unique reads” refer to reads that were mapped to a unique (only one) location of the reference genome
“Multi-mapping reads” refer to reads aligned to more than one locus on the reference genome
Technical samples: CTR1a and CTRb1, FLU1a and FLU1b etc

ISSN: 2049-1891