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Table 1 Summary of data obtained from RNA-Seq of porcine neonatal ovaries

From: Flutamide-induced alterations in transcriptional profiling of neonatal porcine ovaries

Sample RNA Sequencing No.1 RNA Sequencing No.2
CTR1a CTR2a CTR3a FLU1a FLU2a FLU3a CTR1b CTR2b CTR3b FLU1b FLU2b FLU3b
Number of row reads 25,218,729 24,265,954 26,786,205 29,657,600 31,499,003 33,066,267 25,684,749 29,793,840 24,982,931 26,909,112 26,958,364 29,883,752
Number of processed reads 22,946,350 22,025,084 23,919,783 26,265,118 28,431,555 29,051,215 24,815,278 28,489,892 23,409,293 25,573,491 25,420,566 28,108,743
% of processed reads 90.99 90.77 89.30 88.56 90.26 87.86 96.61 95.62 93.70 95.04 94.30 94.06
Unique reads
 Number of uniquely mapped reads 16,920,873 15,737,185 17,549,085 19,080,540 20,774,178 20,886,099 19,415,040 22,389,585 18,221,092 20,157,124 19,841,031 21,895,703
 % of uniquely mapped reads 73.74 71.45 73.37 72.65 73.07 71.89 78.24 78.59 77.84 78.82 78.05 77.90
 Average mapped length, nt 176.97 176.39 177.02 176.83 176.94 176.78 177.53 177.65 177.56 177.60 177.68 177.56
Multi-mapping reads
 Number of reads mapped to multiple loci 1,728,713 1,588,546 1,774,327 1,896,456 2,078,804 2,120,266 1,905,286 2,239,355 1,818,916 1,987,466 1,973,831 2,205,331
 % of reads mapped to multiple loci 7.53 7.21 7.42 7.22 7.31 7.30 7.68 7.86 7.77 7.77 7.76 7.85
Identified transcripts
 Number of identified transcripts 43,478 43,702 41,124 42,171 43,952 43,531 42,672 44,969 40,724 41,812 43,259 43,307
 % of identified transcripts 71.85 72.22 67.96 69.69 72.63 71.93 70.51 74.31 67.29 69.09 71.48 71.56
  1. FLU ovaries from flutamide-treated piglet, CTR ovaries from NaCl-treated piglet
  2. Each biological sample was sequenced two times (for details please see the M & M section)
  3. Sequencing No. 1 (CTR1a, CTR2a, CTR3a, FLU1a, FLU2a and FLU3a) and Sequencing No. 2 (CTR1b, CTR2b, CTR3b, FLU1b, FLU2b and FLU3b) produced two
  4. separate sets of data. The statistics are presented for each sample separately
  5. Preprocessing of data included clipping of adapters, trimming of read ends and removing low quality reads
  6. “Unique reads” refer to reads that were mapped to a unique (only one) location of the reference genome
  7. “Multi-mapping reads” refer to reads aligned to more than one locus on the reference genome
  8. Technical samples: CTR1a and CTRb1, FLU1a and FLU1b etc