Fig. 2

Targeted disruption of the tva gene in DF-1 fibroblasts using the CRISPR/Cas9 system. a The gene structure of tva (CD320) and the locus targeted by CRISPR/Cas9 vectors (TVA#1 and TVA#4). Scale bar = 1 kb. b T7E1 assay of DF-1 cells transfected with the TVA#1 and TVA#4 CRISPR/Cas9 vectors. Wild type (WT) DF-1 fibroblasts were used as the control. c Sequence analysis of transfected DF-1 cells using the TA cloning method. Gray lowercase letters indicate insertions, and gray letters with lines indicate deletions. Indel mutations and their frequencies are presented. Blue bars indicate guide RNA binding sites and red bars indicate protospacer adjacent motif (PAM) sequences