Table 3 Staining reagents used in flow cytometry of immune cells isolated from lungs of milk replacer-fed pigs
Antigen | Clone | Isotype | Fluorochrome | Labeling strategy | Primary antibody source | 2nd Antibody source |
|---|---|---|---|---|---|---|
Immunotyping of myeloid cells | ||||||
 CD14 | TüK4 | IgG2a | FITC | Directly conjugated | ThermoFisher | – |
 CD163 | 2A10/11 | IgG1 | PE | Directly conjugated | ThermoFisher | – |
 CD172A | 74–22-15A | IgG2b | Alexa 647 | Secondary antibody | BEI Resources | Southern Biotech |
Immunotyping of lymphocytes | ||||||
 CD3 | PPT3 | IgG1 | Alexa 488 | Secondary antibodya | BIO RAD | Southern Biotech |
 CD8α | 76–2-11 | IgG2a | Alexa 647 | Secondary antibody | BEI Resources | Southern Biotech |
 CD21a | BB6-11C9.6 | IgG1 | PE-Cy5.5 | Streptavidin-biotin | Novus Biologicals | Southern Biotech |