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Table 3 Staining reagents used in flow cytometry of immune cells isolated from lungs of milk replacer-fed pigs

From: Dietary arachidonate in milk replacer triggers dual benefits of PGE2 signaling in LPS-challenged piglet alveolar macrophages

Antigen Clone Isotype Fluorochrome Labeling strategy Primary antibody source 2nd Antibody source
Immunotyping of myeloid cells
 CD14 TüK4 IgG2a FITC Directly conjugated ThermoFisher
 CD163 2A10/11 IgG1 PE Directly conjugated ThermoFisher
 CD172A 74–22-15A IgG2b Alexa 647 Secondary antibody BEI Resources Southern Biotech
Immunotyping of lymphocytes
 CD3 PPT3 IgG1 Alexa 488 Secondary antibodya BIO RAD Southern Biotech
 CD8α 76–2-11 IgG2a Alexa 647 Secondary antibody BEI Resources Southern Biotech
 CD21a BB6-11C9.6 IgG1 PE-Cy5.5 Streptavidin-biotin Novus Biologicals Southern Biotech
  1. aFree binding sites of secondary antibody were blocked by ChromePure Mouse IgG (Jackson Immuno Research, West Grove, PA) prior to anti-CD21a staining