Jugular arginine supplementation increases lactation performance and nitrogen utilization efficiency in lactating dairy cows

Table 2 Primers of genes for real-time PCR analysis

Gene name	ID number	Sequence (5’to3’)	Product size, bp ^a	Annealing temperature, °C
SLC7A1	NM_001135792	F: TCAACCAGCCTCCTAGCACT	147	60
SLC7A1	NM_001135792	R: GGCACCTTGAATGAGAGCTT	147	60
SLC7A2	XM_010820288	F: CTCGCTCCTCCGTGATAAAT	168	63
SLC7A2	XM_010820288	R: ATTCAGCGATCACCTCCATT	168	63
SLC7A6	NM_001075937	F: GCCTTTGGTTCAGGGTATGC	128	60
SLC7A6	NM_001075937	R: CTGGATAGGTGCCAGCTT	128	60
SLC7A7	NM_001075151	F: GGAGCCTCGACTCACTTTGA	133	60
SLC7A7	NM_001075151	R: GTCCCTCATGTTGAGCACAG	133	60
SLC7A5	NM_173892	F: CTGATGGAGTATGCGAAGCA	164	55
SLC7A5	NM_173892	R: GGGTCCTGGGTCTTTGTGTA	164	55
SLC7A8	NM_001034428	F: CTGCGAACTCATCAGAACCA	134	63
SLC7A8	NM_001034428	R: CTCCAGCAGCCTTTCAGAGT	134	63
ß-actin	NM_173979.3	F:ACTGTTAGCTGCGTTACACCCTT	190	62
ß-actin	NM_173979.3	R: TGCTGTCACCTTCACCGTTCC	190	62
GAPDH	NM_001034034.1	F: GGTCACCAGGGCTGCTTT	176	59
GAPDH	NM_001034034.1	R: CTGTGCCGTTGAACTTGC	176	59

Note: All the primers used in this experiment were synthesized in Invitrogen (Nanjing, China)
^abp = bases of pairs
Genes: GAPDH = glyceraldehyde-3-phosphate dehydrogenase; SLC7A1 = solute carrier family 7 member 1; SLC7A2 = solute carrier family 7 member 2; SLC7A5 = solute carrier family 7 member 5; SLC7A6 = solute carrier family 7 member 6; SLC7A7 = solute carrier family 7 member 7; SLC7A8 = solute carrier family 7 member 8

ISSN: 2049-1891