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Table 2 Time-dependent effect of MNP-lectin conjugates on sperm motility characteristics

From: Nanotechnology-based approach for safer enrichment of semen with best spermatozoa

Other motion

Incubation time, min

P-values

parameters

0

(N=18)

30

(N=19)

90

(N=15)

120

(N=16)

VAP, μm/s

70 ± 2a

77.6 ± 2.1b

73.2 ± 3.1c

74.8 ± 1.7bc

< 0.0001

VSL, μm/s

35.4 ± 1.1a

40.5 ± 0.8b

45.1 ± 0.7c

42.7 ± 1.3d

< 0.0001

VCL, μm/s

154.5 ± 4.1a

164.7 ± 4.3b

150.3 ± 4.8a

160.4 ± 2.9b

0.001

ALH, μm

8.3 ± 0.1a

7.7 ± 0.1b

7.2 ± 0.3c

7.9 ± 0.1b

< 0.0001

BCF, Hz

38.1 ± 0.4a

36.2 ± 0.5b

37.9 ± 0.9a

34.1 ± 0.6c

< 0.0001

STR, %

51.1 ± 0.6a

54.2 ± 0.9b

63.1 ± 2.5c

58.1 ± 1.0d

< 0.0001

LIN, %

24.3 ± 0.5a

26.9 ± 0.5b

33.1 ± 2.2c

28.2 ± 0.6b

< 0.0001

  1. The table represents various sperm kinematic parameters, such as average path velocity (VAP), straight-line velocity (VSL), curve-linear velocity (VCL), head lateral amplitude (ALH), beat flagellum frequency (BCF), straightness (STR – VSL/VAP × 100), linearity (LIN – VSL/VCL × 100). Data are mean (±sem) of N observations of at least 4 independent replicates using pooled semen doses. Different letters on the same line denote significant differences (P<0.05)