Surplus dietary isoleucine intake enhanced monounsaturated fatty acid synthesis and fat accumulation in skeletal muscle of finishing pigs

Table 2 Primers used for real-time qPCR

Gene^a	Primers	Primer sequences (5′→3′)	Size, bp	Tm, °C	Accession No.
ADD1	Forward	GCGACGGTGCCTCTGGTAGT	218	60	XM_021066226.1
ADD1	Reverse	CGCAAGACGGCGGATTTA	218	60	XM_021066226.1
FABP4	Forward	TGGAAACTTGTCTCCAGTG	147	54	NM_001002817.1
FABP4	Reverse	GGTACTTTCTGATCTAATGGTG	147	54	NM_001002817.1
FAS	Forward	GCCTAACTCCTCGCTGCAAT	195	60	NM_001099930.1
FAS	Reverse	TCCTTGGAACCGTCTGTGTTC	195	60	NM_001099930.1
HSL	Forward	TCGGAGTGAACGGATTTG	195	60	–
HSL	Reverse	TCCTCCTTGGTGCTAATCTCGT	195	60	–
LPL	Forward	CTCGTGCTCAGATGCCCTAC	148	60	NM_214286.1
LPL	Reverse	GGCAGGGTGAAAGGGATGTT	148	60	NM_214286.1
SCD	Forward	GCCTACTATCTGCTGAGTGC	152	57	XM_021072070.1
SCD	Reverse	TCTCGGGCCCATTCATAAAC	152	57	XM_021072070.1
GAPDH	Forward	TCGGAGTGAACGGATTTG	219	60	NM_001206359.1
GAPDH	Reverse	CCTGGAAGATGGTGATGG	219	60	NM_001206359.1

^aADD1 Adipocyte determination and differentiation factor 1, FABP4 Fatty acid binding protein 4, FAS Fatty acid synthase, HSL Hormone sensitive lipase, LPL Lipoprotein lipase, SCD Stearoyl-CoA desaturase, GAPDH Glyceraldehyde-3-phosphate dehydrogenase

ISSN: 2049-1891