Fig. 5
From: Effects of galacto-oligosaccharides on growth and gut function of newborn suckling piglets
The relative mRNA and protein expression of the jejunal tight junction in suckling piglets. Piglets assigned to CON (n = 6) and GOS (n = 6) received physiological saline and GOS solution for 7 d after birth, respectively. (a-b) The relative mRNA expression of the jejunal tight junction in suckling piglets. The values were calculated relative to the expression of β-actin with formula 2-∆∆Ct. (c) The blots of zonula occludens-1 (ZO-1), occludin, and β-actin of the jejunum mucosa in suckling piglets. (d-e) The relative protein expressions of the jejunal tight junction in suckling piglets. The value of protein expression was the ratio of the densitometry units of tight junction protein to β-actin. Values are expressed as means ± SD. Bars assigned with different lower-case letters indicate significant differences. CON: control group; GOS: GOS group