Table 3 RT-PCR Primer sequences of target and reference genes
Genes | Primer sequence (5′→3′) | Product, bp | GenBank No. |
|---|---|---|---|
TLR2 | F:TCGAAAAGAGCCAGAAAACCAT | 58 | NM213761 |
R:CTTGCACCACTCGCTCTTCA | Â | ||
TLR4 | F:AGAAAATATGGCAGAGGTGAAAGC | 64 | GQ304754 |
R:CTTCGTCCTGGCTGGAGTAGA | Â | ||
NF-κB | F:TGCTGGACCCAAGGACATG | 60 | AK348766.1 |
R:CTCCCTTCTGCAACAACACGTA | Â | ||
IL-6 | F:GATGCTTCCAATCTGGGTTCA | 62 | M80258.1 |
R:CACAAGACCGGTGGTGATTCT | Â | ||
IL-10 | F: GCCTTCGGCCCAGTGAA | 71 | NM214041.1 |
R: AGAGACCCGGTCAGCAACAA | Â | ||
TNF-α | F: TCTATTTTGGGATCATTGCCC | 127 | NM214022.1 |
R: CCAGCCCCTCATTCTCTTTCT | Â | ||
β-actin | F:GGCGCCCAGCACGAT | 66 | DQ845171.1 |
R:CCGATCCACACGGAGTACTTG | Â |