Alginate oligosaccharide-induced intestinal morphology, barrier function and epithelium apoptosis modifications have beneficial effects on the growth performance of weaned pigs

Table 2 Primer sequences for quantitative real-time polymerase chain reaction

Gene^a	Primer sequence (5′→3′)	Size, bp	Accession No.
MUC1	Forward: GTGCCGCTGCCCACAACCTG Reverse: AGCCGGGTACCCCAGACCCA	141	XM_001926883.4
MUC2	Forward: GGTCATGCTGGAGCTGGACAGT Reverse: TGCCTCCTCGGGGTCGTCAC	181	XM_013989745.1
MUC4	Forward: GATGCCCTGGCCACAGAA Reverse: TGATTCAAGGTAGCATTCATTTGC	89	XM_001926442.1
BAX	Forward: CTGACGGCAACTTCAACTGG Reverse: CGTCCCAAAGTAGGAGAGGA	200	XM_003127290.4
BCL2	Forward: AGCATGCGGCCTCTATTTGA Reverse: GGCCCGTGGACTTCACTTAT	120	XM_003121700.2
FAS	Forward: TGATGCCCAAGTGACTGACC Reverse: GCAGAATTGACCCTCACGAT	103	NM_213839.1
caspase-3	Forward: GTGGGACTGAAGATGACA Reverse: ACCCGAGTAAGAATGTG	190	NM_214131.1
caspase-8	Forward: AGACAAGGGCATCATCATCGG Reverse: GGTTTACCAAGAAGGGAACGG	102	NM_001031779.2
caspase-9	Forward: AATGCCGATTTGGCTTACGT Reverse: CATTTGCTTGGCAGTCAGGTT	195	XM_003127618.4
GAPDH	Forward: ATGGTGAAGGTCGGAGTGAAC Reverse: CTCGCTCCTGGAAGATGGT	235	NM_001206359.1

^aMUC1, mucin 1; MUC2, mucin 2; MUC4, mucin 4; BAX, B-cell lymphoma-2-associated X protein; BCL2, B-cell lymphoma-2; caspase-3, cysteinyl aspartate-specific proteinase-3; caspase-8, cysteinyl aspartate-specific proteinase-8; caspase-9, cysteinyl aspartate-specific proteinase-9; GAPDH, glyceraldehyde-3-phosphate dehydrogenase

ISSN: 2049-1891