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Table 2 Primer sequences for quantitative real-time polymerase chain reaction

From: Alginate oligosaccharide-induced intestinal morphology, barrier function and epithelium apoptosis modifications have beneficial effects on the growth performance of weaned pigs

Genea Primer sequence (5′→3′) Size, bp Accession No.
MUC1 Forward: GTGCCGCTGCCCACAACCTG Reverse: AGCCGGGTACCCCAGACCCA 141 XM_001926883.4
MUC2 Forward: GGTCATGCTGGAGCTGGACAGT Reverse: TGCCTCCTCGGGGTCGTCAC 181 XM_013989745.1
MUC4 Forward: GATGCCCTGGCCACAGAA Reverse: TGATTCAAGGTAGCATTCATTTGC 89 XM_001926442.1
BAX Forward: CTGACGGCAACTTCAACTGG Reverse: CGTCCCAAAGTAGGAGAGGA 200 XM_003127290.4
BCL2 Forward: AGCATGCGGCCTCTATTTGA Reverse: GGCCCGTGGACTTCACTTAT 120 XM_003121700.2
FAS Forward: TGATGCCCAAGTGACTGACC Reverse: GCAGAATTGACCCTCACGAT 103 NM_213839.1
caspase-3 Forward: GTGGGACTGAAGATGACA Reverse: ACCCGAGTAAGAATGTG 190 NM_214131.1
caspase-8 Forward: AGACAAGGGCATCATCATCGG Reverse: GGTTTACCAAGAAGGGAACGG 102 NM_001031779.2
caspase-9 Forward: AATGCCGATTTGGCTTACGT Reverse: CATTTGCTTGGCAGTCAGGTT 195 XM_003127618.4
GAPDH Forward: ATGGTGAAGGTCGGAGTGAAC Reverse: CTCGCTCCTGGAAGATGGT 235 NM_001206359.1
  1. aMUC1, mucin 1; MUC2, mucin 2; MUC4, mucin 4; BAX, B-cell lymphoma-2-associated X protein; BCL2, B-cell lymphoma-2; caspase-3, cysteinyl aspartate-specific proteinase-3; caspase-8, cysteinyl aspartate-specific proteinase-8; caspase-9, cysteinyl aspartate-specific proteinase-9; GAPDH, glyceraldehyde-3-phosphate dehydrogenase