Ileum terminal antibiotic infusion affects jejunal and colonic specific microbial population and immune status in growing pigs

Table 2 List of primers used in this study

Bacteria	Forward primer, 5′→3’	Reverse primer, 5′→3’	Reference	Annealing temp, °C
Total bacteria	GTGSTGCAYGGYYGTCGTCA	ACGTCRTCCMCNCCTTCCTC	[63]	60
Bacteroides-Prevotella	GAGAGGAAGGTCCCCCAC	CGCTACTTGGCTGGTTCAG	[64]	60
Bifidobacterium	TCGCGTCYGGTGTGAAAG	GGTGTTCTTCCCGATATCTACA	[65]	60
Clostridium cluster IV	GCACAAGCAGTGGAGT	CTTCCTCCGTTTTGTCAA	[66]	60
Clostridium cluster XIVa	CGGTACCTGACTAAGAAGC	AGTTTYATTCTTGCGAACG	[67]	60
Escherichia coli	CATGCCGCGTGTATGAAGAA	CGGGTAACGTCAATGAGCAAA	[68]	60
Lactobacillus	AGCAGTAGGGAATCTTCCA	ATTCCACCGCTACACATG	[69]	60

ISSN: 2049-1891