Fig. 3

miR-25 represses intracellular triglyceride content and lipid droplet accumulation in GMECs. a Quantitative real-time PCR was performed to measure the expression level of miR-25 in GMECs 48 h after transfection with miR-25 mimics (miR-25), compared with mimic control (miR-NC). miR-17 was used as a positive control. The error bars depict the mean ± S.D. of at least three independent experiments. *P < 0.05. b Chemical analysis showing miR-25 overexpression leading to decreased triglyceride levels in GMECs. Data are shown as the mean ± S.D. of three independent cell samples. **P < 0.01. c Analysis of the presence of lipid droplets in GMECs treated for 48 h with or without miR-25 mimics followed by Oil Red O staining. Scale bar, 200 μm. d Oil Red O-based quantification of fat accumulation in GMECs. Each bar represents the mean ± S.D. for three samples. *P < 0.05