Interferon-γ acts as a regulator in the trade-off between phagocytosis and production performance in dwarf chickens

Table 1 Real-time PCR primers sequences for the target genes

Gene^a	Accession No.	Primer Sequences^b (5′→3′)	Product size, bp	Temperature, °C
GAPDH	NM_204305.1	F: CTGAGAACGGGAAACTTGTG	105	60
GAPDH	NM_204305.1	R: CCACAACATACTCAGCACCTG	105	60
FSH-β	NM_204257.1	F: AGCAGTGGAAAGAGAAGAATGTGA	151	60
FSH-β	NM_204257.1	R: TGTTTCATACACAACCTCCTTGAAG	151	60
IFNGR1	NM_001130387.1	F: AACCTGAGCATCCCAGTTCC	135	60
IFNGR1	NM_001130387.1	R: ACTCCAAGCCTGCGTGATAG	135	60
IFNGR2	NM_001008676.2	F: GCACCGGAGGATGTAATGGT	145	60
IFNGR2	NM_001008676.2	R: GGGTGCAGTCCATCTCACTC	145	60

^aGAPDH glyceraldehyde-3-phosphate dehydrogenase, FSH-β follicle stimulating hormone beta subunit, IFNGR1 interferon gamma receptor 1, IFNGR2 interferon gamma receptor 2
^bF, forward; R, reverse

ISSN: 2049-1891