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Table 2 Effect of gas stunning methods on serum variables in broilers

From: Gas stunning with CO2 affected meat color, lipid peroxidation, oxidative stress, and gene expression of mitogen-activated protein kinases, glutathione S-transferases, and Cu/Zn-superoxide dismutase in the skeletal muscles of broilers

Serum variables2

Stunning methods1

P-value

Control

G40%

G79%

 

Cortisol, ng/mL

2.21 ± 0.23 a

3.72 ± 0.33 b

2.33 ± 0.21 a

< 0.01

Uric acid, mg/mL

205 ± 17.8 a

304 ± 26.2 b

298 ± 13.1 b

< 0.01

FT3, fmol/mL

2.29 ± 0.40

1.24 ± 0.14

1.82 ± 0.44

0.14

FT4, fmol/mL

3.14 ± 0.50 a

5.21 ± 0.79 b

3.74 ± 0.41 ab

0.07

T3, ng/mL

1.11 ± 0.08 b

0.67 ± 0.03 a

0.85 ± 0.06 ab

0.03

T4, ng/mL

38.5 ± 2.03

41.4 ± 4.00

41.2 ± 4.43

0.51

T3/T4, %

2.87 ± 0.20 b

1.56 ± 0.12 a

2.26 ± 0.25 ab

0.06

FT3/FT4, %

77.3 ± 7.4 c

24.7 ± 2.73 a

49.3 ± 4.8 b

<  0.01

  1. 1Control = without stunning; G40% = 40% CO2 + 21% O2 + 39%N2; G79% = 79% CO2 + 21% O2
  2. 2FT3, FT4, T3, T4 represent free triiodothyronine, free thyroxine, triiodothyronine, and thyroxine, respectively
  3. a–bMeans with no common superscripts within a row differ significantly (P ≤ 0.05) or exhibit differences that approach significance (0.05 < P < 0.10); n = 6; values are expressed as the means ± S.E.M