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Fig. 5 | Journal of Animal Science and Biotechnology

Fig. 5

From: Acquisition of pluripotency in the chick embryo occurs during intrauterine embryonic development via a unique transcriptional network

Fig. 5

Pluripotency-related genes expression during the chicken developmental stage of pluripotency acquisition. a Chicken embryonic stem cell-related gene cENS-1, b naive pluripotency-related genes cTFCP2L1, cTBX3, and cPRDM14, and c general pluripotency-related genes cKIT, cLIN28A, cMYC, and cSALL4 expression in oocyte and intrauterine chicken embryos were analyzed using qRT-PCR. cGAPDH was used as a reference gene. Results are shown as mean ± standard error of mean (n = 3). Significant differences of the relative gene expression between consecutive developmental stages (Oocyte vs. EGK.I, EGK.I vs. EGK.III, EGK.III vs. EGK.VI, EGK.VI vs. EGK.VIII, and EGK.VIII vs. EGK.X) were represented as ** P < 0.01, *** P < 0.001 and **** P < 0.0001

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