Dietary proline supplementation alters colonic luminal microbiota and bacterial metabolite composition between days 45 and 70 of pregnancy in Huanjiang mini-pigs

Table 5 Short-chain fatty acid concentrations in colonic contents of pregnant Huanjiang mini-pigs (mg/g)

Item	Control group		Proline group		SEM	P-values
Item	45 d	70 d	45 d	70 d	SEM	Diet	Day	Diet × Day
Proximal colonic contents
Acetate	16.72	34.24	14.70	22.76	1.07	0.02	< 0.01	0.099
Propionate	5.95	12.56	6.36	9.44	0.71	0.28	< 0.01	0.16
Isobutyrate	0.23	0.92	0.28	0.71	0.19	0.41	< 0.01	0.17
Butyrate	3.64	6.69	3.68	4.18	0.51	0.06	0.010	0.05
Isovalerate	0.19	0.70	0.21	0.50	0.16	0.15	< 0.01	0.09
Valerate	0.43	1.43	0.44	1.16	0.24	0.35	< 0.01	0.34
Total BCFA	0.42	1.61	0.49	1.21	0.25	0.28	< 0.01	0.13
Total straight-chain fatty acids	26.75	54.92	25.18	37.54	1.27	0.02	< 0.01	0.06
Total SCFA	27.17	56.53	25.67	38.74	1.29	0.03	< 0.01	0.05
Distal colonic contents
Acetate	10.48	9.07	10.38	10.02	0.60	0.60	0.28	0.52
Propionate	4.01	3.81	4.00	4.60	0.38	0.24	0.54	0.24
Isobutyrate	0.36	0.49	0.34	0.48	0.13	0.78	0.01	0.92
Butyrate	2.94	2.95	2.93	2.71	0.38	0.71	0.75	0.73
Isovalerate	0.33	0.39	0.31	0.34	0.13	0.34	0.25	0.62
Valerate	0.48	0.50	0.41	0.51	0.14	0.57	0.17	0.39
Total BCFA	0.69	0.88	0.66	0.82	0.18	0.53	0.03	0.84
Total straight-chain fatty acids	17.90	16.33	17.72	17.85	0.76	0.61	0.59	0.52
Total SCFA	18.59	17.21	18.38	18.67	0.77	0.64	0.69	0.54

ISSN: 2049-1891