Fig. 1

Dolichos biflorus agglutinin (DBA) immunostaining of neonatal gonocytes. a–c Gonocyte immunostaining performed on testis cell smears collected immediately following cell isolation. d–f Multiple gonocytes form a colony on a somatic-cell monolayer observed at 1 wk of culture. g–i Floating gonocytes in the removed medium observed 4 d after incubation. b, e, h Fluorescein-conjugated DBA-labeled gonocytes appear green under the fluorescence microscope. a, d, g DAPI-stained nuclei of the cells appear blue. c, f, i Merged images