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Table 1 Kinetic parameters of CHO cells transfected with bovine, sheep, pig, and chicken PepT1 cDNA

From: Functional characterization of oligopeptide transporter 1 of dairy cows

Substrate Molecular weight Chargea Hydrop-hobicityb pI IC50, mmol/L
Bovine Sheepc Pigd Chickene
Gly-Lys 203 Positive 3.3 10.1 0.078 ND ND ND
Gly-Met 206 Neutral 1.5 5.5 0.032 ND 0.03 0.07
Lys-Lys 274 Positive −7.8 10.0 9.649 0.739 3.8 7.9
Lys-Met 277 Positive −2.0 8.8 0.032 0.051 0.04 0.11
Lys-Phe 293 Positive −1.1 8.8 0.090 0.024 0.03 0.11
Met-Glu 278 Negative −1.6 4.6 0.023 0.037 0.53 0.02
Met-Gly 206 Neutral 1.5 5.3 0.058 0.016 0.008 0.27
Met-Leu 262 Neutral 5.7 5.3 0.080 0.021 0.004 0.04
Met-Lys 277 Positive −2.0 8.5 0.020 0.123 ND 0.07
Met-Met 280 Neutral 3.8 5.3 0.096 0.024 0.013 0.02
Trp-Phe 351 Neutral 1.9 5.5 0.014 ND 0.06 0.02
Leu-Gly-Gly 245 Neutral 3.0 5.5 2.069 0.127 0.27 0.08
Lys-Trp-Lys 460 Positive −8.7 10.0 CBD 3.732 2.2 5.9
Met-Leu-Phe 409 Neutral 8.5 5.3 0.050 0.014 0.4 0.04
Met-Gly-Met-Met 468 Neutral 5.3 5.3 6.043 0.952 CBD 3.9
  1. pI isoelectric point, calculated using pK values of the constituent amino acids, IC 50 50% inhibitory concentration, ND not determined; CBD cannot be determined
  2. a Charge was calculated at pH 6.0
  3. b Hydrophobicity was calculated as the average of the value of the constituent amino acids according to Kyte and Doolittle [38]
  4. c Sheep PepT1 data from Chen et al. [14]
  5. d Pig PepT1 data from Klang et al. [17]
  6. e Chicken PepT1 data from Chen et al. [15]