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Table 1 Embryonic development and blastocyst formation after 144 h IVC with SIRT1 activators or inhibitors

From: SIRT1-dependent modulation of methylation and acetylation of histone H3 on lysine 9 (H3K9) in the zygotic pronuclei improves porcine embryo development

  No. of fertilized oocytes No. of cleaved oocytes (mean % ± SEM) No. of blastocysts (mean % ± SEM) Mean cell No. per blastocyst (mean ± SEM)
DMSO, % (v/v) 0.5 38 28 (65.9 ± 8.3)a 2 (5.2 ± 2.9)bc 36.0 ± 5.0a
Resveratrol, μmol/L 3 67 47 (67.7 ± 10.6)a 4 (6.2 ± 2.8)bc 39.3 ± 11.3a
6.25 51 36 (69.9 ± 6.4)a 0 (0.0 ± 0.0)c
12.5 50 20 (39.2 ± 5.2)b 0 (0.0 ± 0.0)c
Nicotinamide, mmol/L 2.5 50 39 (76.7 ± 6.9)a 7 (16.7 ± 8.8)abc 30.9 ± 4.2a
5 70 45 (64.1 ± 1.4)a 7 (9.7 ± 6.2)bc 36.7 ± 4.5a
7.5 69 45 (66.7 ± 4.6)a 13 (21.4 ± 8.4)ab 34.9 ± 3.1a
BML-278, μmol/L 3 68 44 (62.7 ± 7.1)a 18 (32.9 ± 8.1)a 38.4 ± 4.2a
Sirtinol, μmol/L 10 69 44 (63.5 ± 4.6)a 4 (6.5 ± 3.7)bc 23.5 ± 1.3a
  1. a,b,cDifferent superscripts within the same column were significantly different at P ≤ 0.05