SIRT1-dependent modulation of methylation and acetylation of histone H3 on lysine 9 (H3K9) in the zygotic pronuclei improves porcine embryo development

Table 1 Embryonic development and blastocyst formation after 144 h IVC with SIRT1 activators or inhibitors

		No. of fertilized oocytes	No. of cleaved oocytes (mean % ± SEM)	No. of blastocysts (mean % ± SEM)	Mean cell No. per blastocyst (mean ± SEM)
DMSO, % (v/v)	0.5	38	28 (65.9 ± 8.3)^a	2 (5.2 ± 2.9)^bc	36.0 ± 5.0^a
Resveratrol, μmol/L	3	67	47 (67.7 ± 10.6)^a	4 (6.2 ± 2.8)^bc	39.3 ± 11.3^a
	6.25	51	36 (69.9 ± 6.4)^a	0 (0.0 ± 0.0)^c	–
	12.5	50	20 (39.2 ± 5.2)^b	0 (0.0 ± 0.0)^c	–
Nicotinamide, mmol/L	2.5	50	39 (76.7 ± 6.9)^a	7 (16.7 ± 8.8)^abc	30.9 ± 4.2^a
	5	70	45 (64.1 ± 1.4)^a	7 (9.7 ± 6.2)^bc	36.7 ± 4.5^a
	7.5	69	45 (66.7 ± 4.6)^a	13 (21.4 ± 8.4)^ab	34.9 ± 3.1^a
BML-278, μmol/L	3	68	44 (62.7 ± 7.1)^a	18 (32.9 ± 8.1)^a	38.4 ± 4.2^a
Sirtinol, μmol/L	10	69	44 (63.5 ± 4.6)^a	4 (6.5 ± 3.7)^bc	23.5 ± 1.3^a

^a,b,cDifferent superscripts within the same column were significantly different at P ≤ 0.05

ISSN: 2049-1891