Table 1 The specific primers for qPCR in this study
From: The regulation of IMF deposition in pectoralis major of fast- and slow- growing chickens at hatching
Gene | Sequence | Product size | Accession NO. |
|---|---|---|---|
THRSP | F:5′-ATCAAGCCCGTGGTGGAGC-3′ R:5′-CTTTGGTGTTTTTGGTGAGGTCG-3’ | 184 bp | NM_213577 |
ACACA | F:5’-AACCTGCTAAACCCCTGG-3′ R:5′-AGTCCCAAATCCGAAAGG-3’ | 175 bp | NM_205505 |
ACSS1 | F:5’-TGGGAGATGTTACCACAC-3′ R:5′-GCAGAATACACCAAGAGAG-3’ | 181 bp | XM_415011 |
PPARG | F:5’-TAAAGTCCTTCCCGCTGACCAAA-3′ R:5′-AAATTCTGTAATCTCCTGCACTGCCTC-3’ | 230 bp | NM_001001460 |
LPL | F: 5’-AGGAGAAGAGGCAGCAATA-3′ R:5′-AAAGCCAGCAGCAGATAAG-3’ | 222 bp | AB016987 |
FABP4 | F:5’-GGGGTTTGCTACCAGGAAGATG-3′ R:5′-CATTCCACCAGCAGGTTCCC-3’ | 276 bp | NM_204290 |
RBP7 | F: 5’-TTCCATCCATACCACAAGCACA-3′ R:5′-AGTGAGTCCAGCCCCTGTTCTT-3’ | 179 bp | XM_417606 |
DGAT2 | F: 5’-AATGGGTCCTCACGTTCC-3′ R:5′-TGGTGGTCAGCAGGTTGT-3’ | 237 bp | XM_419374 |
GK | F: 5’-TATGGCTGCTACTTTGTGC-3′ R:5′-GTATCCCGCAGTCCTTGT-3’ | 187 bp | XM_416788 |
ACAT1 | F: 5’-CTCCAGCAAGACAGGCAGT-3′ R:5′-CACCAGCAACCATTACATCC-3’ | 150 bp | XM_417162 |
ACOX2 | F: 5’-TATGTAAGGCGTGGGTCA-3′ R:5′-TATGTAAGGCGTGGGTCA-3’ | 198 bp | XM_414406 |
ACOX3 | F: 5’-ACATCTGGCTGTGCTCTATC-3′ R:5′-ACTCCCCGCTAGCTTTAC-3’ | 179 bp | XM_420814 |
CPT1A | F: 5’-AGACGGACACTGCAAAGGAG-3′ R:5′-AGCCCCTTCCCAAAAACA-3’ | 174 bp | NM_001012898 |
CPT2 | F: 5’-GGGTCGTGTTGGGCTGTT-3′ R:5′-AAAGAGGTTTCTGGGCGTTC-3’ | 168 bp | XM_001234342 |
DAK | F:5’-AGAGGAGGAAGGAATTGACCTC-3′ R:5′-GTCGAAGACCACATGGCTGT-3’ | 272 bp | NM_001079500 |
APOO | F: 5’-CTGCCTTCTGCCTCAGGAAA-3′ R:5′-CAATGCTGATCCTGCAACGG-3’ | 162 bp | XM_015272548 |
FUT9 | F: 5’-TGAAATGTGTAGCTGCGTGGA-3′ R:5′-AGACGTCTCCGAATTGCTTGT-3’ | 141 bp | NM_001079502 |
GCNT1 | F: 5’-ACCAAGATACTGGAGGGCGA-3′ R:5′-CTCACTGCTGAGAGGTTCCA-3’ | 174 bp | XM_003643022 |
B4GALT3 | F:5’-TCCTCCTGCACGATGTGAAC-3′ R:5′-TCGCCCCAGTATGTGTTTGG-3’ | 202 bp | XM_416564 |