Fig. 2
Sequential purification steps of recombinant OVGP1 (ROVGP1). Chromatogram of (a) IMAC based affinity purification: purification of recombinant OVGP1 through IMAC resulted in a single peak (indicated by arrow) after applying a gradient of 0–100% (300 mmol/L imidazole) in 30 mL. The peak fractions contained partially purified recombinant OVGP1. b Ion-Exchange Chromatography: Purification step through IEX showing elution of three merged peaks (indicated by arrows) during a stepwise gradient run. The latter peak contained recombinant OVGP1 with a few remaining contaminating proteins. c Size-Exclusion Chromatography: Purification of recombinant OVGP1 through the step resulted in elution of a peak (indicated by arrow) containing purified recombinant OVGP1